4fr5

X-ray diffraction
2.2Å resolution

Crystal Structure of Shikimate Dehydrogenase (aroE) Y210S Mutant from Helicobacter pylori in Complex with Shikimate

Released:
Source organism: Helicobacter pylori 26695
Entry authors: Cheng WC, Chen TJ, Wang WC

Function and Biology Details

Reaction catalysed:
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shikimate dehydrogenase (NADP(+)) Chains: A, B
Molecule details ›
Chains: A, B
Length: 271 amino acids
Theoretical weight: 30.31 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P56119 (Residues: 1-263; Coverage: 100%)
Gene names: HP_1249, aroE
Sequence domains: Shikimate dehydrogenase substrate binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P212121
Unit cell:
a: 46.507Å b: 88.523Å c: 118.185Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.231
Expression system: Escherichia coli BL21(DE3)