› 4fo8

X-ray diffraction
1.9Å resolution

Pseudomonas aeruginosa MetAP with Met, in Mn form

Released:
DOI: 10.2210/pdb4fo8/pdb
PDB entry 4fo8 coloured by chain, front view.
PDB entry 4fo8 coloured by chain, side view.
PDB entry 4fo8 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Ye QZ, Lu JP

Function and Biology Details

Reaction catalysed: 
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine aminopeptidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 280 amino acids
Theoretical weight: 31.39 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HXY1 (Residues: 1-261; Coverage: 100%)
Gene names: PA3657, map
Sequence domains: Metallopeptidase family M24
Structure domains: Creatinase/methionine aminopeptidase superfamily

Ligands and Environments

2 bound ligands:
Ligand MN 13 x MN
Ligand MET 4 x MET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 80.651Å b: 105.664Å c: 133.155Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.2
Expression system: Escherichia coli BL21(DE3)

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