4flt

X-ray diffraction
2.9Å resolution

Pyrococcus abyssi B family DNA polymerase bound to a dsDNA, in edition mode

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase 1 Chain: A
Molecule details ›
Chain: A
Length: 793 amino acids
Theoretical weight: 91.81 KDa
Source organism: Pyrococcus abyssi GE5
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CL77 (Residues: 1-771; Coverage: 100%)
Gene names: PAB1128, PYRAB17200, pol, polI
Sequence domains:
Structure domains:
Primer strand Chain: P
Molecule details ›
Chain: P
Length: 11 nucleotides
Theoretical weight: 3.4 KDa
Source organism: synthetic construct
Expression system: Not provided
Template strand Chain: T
Molecule details ›
Chain: T
Length: 13 nucleotides
Theoretical weight: 4.03 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 68.93Å b: 114.4Å c: 128.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.206
Expression systems:
  • Escherichia coli
  • Not provided