4fda

X-ray diffraction
2.1Å resolution

Crystal structure of Saccharomyces cerevisiae 3-oxoacyl-[acyl-carrier-protein] reductase complexed with NADP

Released:
Entry authors: Yujie Z, Yongxiang G

Function and Biology Details

Reaction catalysed:
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] reductase Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 30.7 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P35731 (Residues: 1-273; Coverage: 98%)
Gene names: OAR1, YKL055C
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 128.73Å b: 128.73Å c: 40.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.229
Expression system: Escherichia coli