› 4fbi

X-ray diffraction
1.5Å resolution

Crystal Structure of an R46A mutant of the Restriction-Modification Controller Protein C.Esp1396I (Trigonal Form)

Released:
DOI: 10.2210/pdb4fbi/pdb
PDB 4fbi coloured by chain and viewed from the front.
PDB 4fbi coloured by chain and viewed from the side.
PDB 4fbi coloured by chain and viewed from the top.
Source organism: Enterobacter sp. RFL1396
Primary publication:
Structural and mutagenic analysis of the RM controller protein C.Esp1396I.
Martin RN, McGeehan JE, Kneale G
OpenAccess logo PLoS One 9 e98365 (2014)
PMID: 24887147

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH cro/C1-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 82 amino acids
Theoretical weight: 9.44 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GGH0 (Residues: 1-79; Coverage: 100%)
Gene name: esp1396IC
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P32
Unit cell:
a: 65.329Å b: 65.329Å c: 71.38Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.199
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Temporal dynamics of methyltransferase and restriction endonuclease accumulation in individual cells after introducing a restriction-modification system.
Morozova et al. (2016)

1 mention without citation

Structural and mutagenic analysis of the RM controller protein C.Esp1396I.
Martin et al. (2014)