X-ray diffraction
2.81Å resolution

Crystal Structure of VldE, the pseudo-glycosyltransferase, in complex with GDP and Trehalose


Function and Biology Details

Reaction catalysed:
GDP-valienol + validamine 7-phosphate = validoxylamine A 7'-phosphate + GDP
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Validamine 7-phosphate valienyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 497 amino acids
Theoretical weight: 55.01 KDa
Source organism: Streptomyces hygroscopicus subsp. limoneus
Expression system: Escherichia coli
  • Canonical: Q15JG1 (Residues: 1-497; Coverage: 100%)
Gene names: SHL15_8006, vldE
Sequence domains: Glycosyltransferase family 20
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 320.821Å b: 122.823Å c: 93.857Å
α: 90° β: 91.62° γ: 90°
R R work R free
0.214 0.211 0.258
Expression system: Escherichia coli