X-ray diffraction
2Å resolution

Structure of Isopropylmalate dehydrogenase from Thermus thermophilus in complex with IPM, Mn and NADH


Function and Biology Details

Reaction catalysed:
(1a) (2R,3S)-3-isopropylmalate + NAD(+) = (2S)-2-isopropyl-3-oxosuccinate + NADH
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
3-isopropylmalate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 359 amino acids
Theoretical weight: 38.4 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
  • Canonical: Q5SIY4 (Residues: 1-345; Coverage: 100%)
Gene names: TTHA1230, leuB
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

Cofactor: Ligand NAD 4 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2
Unit cell:
a: 148.38Å b: 50.72Å c: 178.24Å
α: 90° β: 93.09° γ: 90°
R R work R free
0.152 0.152 0.196
Expression system: Escherichia coli