4f6n

X-ray diffraction
2.8Å resolution

Crystal structure of Kaiso zinc finger DNA binding protein in complex with methylated CpG site DNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional regulator Kaiso Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 16.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86T24 (Residues: 472-604; Coverage: 20%)
Gene names: KAISO, ZBTB33, ZNF348
Structure domains: Classic Zinc Finger
DNA (5'-D(*GP*TP*GP*TP*CP*AP*CP*(5CM)P*GP*(5CM)P*GP*TP*CP*TP*AP*TP*AP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.82 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*GP*TP*AP*TP*AP*GP*AP*(5CM)P*GP*(5CM)P*GP*GP*TP*GP*AP*CP*AP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 19 nucleotides
Theoretical weight: 5.88 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: F222
Unit cell:
a: 44.049Å b: 183.402Å c: 230.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.261
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided