› 4f59

X-ray diffraction
1.71Å resolution

Triple mutant Src SH2 domain

Released:
DOI: 10.2210/pdb4f59/pdb
PDB entry 4f59 coloured by chain, front view.
PDB entry 4f59 coloured by chain, side view.
PDB entry 4f59 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Superbinder SH2 domains act as antagonists of cell signaling.
Kaneko T, Huang H, Cao X, Li X, Li C, Voss C, Sidhu SS, Li SS
Sci Signal 5 ra68 (2012)
PMID: 23012655

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 12.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12931 (Residues: 144-252; Coverage: 20%)
Gene names: SRC, SRC1
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P61
Unit cell:
a: 67.07Å b: 67.07Å c: 46.91Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Cargo recognition in clathrin-mediated endocytosis.
Traub et al. (2013)
8 more

1 mention without citation

Engineered SH2 Domains for Targeted Phosphoproteomics.
Martyn et al. (2022)