› 4f3c

X-ray diffraction
1.93Å resolution

Crystal structure of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Salmonella enterica with butyl-thio-DADMe-Immucillin-A

Released:
DOI: 10.2210/pdb4f3c/pdb
PDB entry 4f3c coloured by chain, front view.
PDB entry 4f3c coloured by chain, side view.
PDB entry 4f3c coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Choleraesuis str. SCSA50
Primary publication:
Salmonella enterica MTAN at 1.36 Å resolution: a structure-based design of tailored transition state analogs.
Haapalainen AM, Thomas K, Tyler PC, Evans GB, Almo SC, Schramm VL
Structure 21 963-74 (2013)
PMID: 23685211

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 26.32 KDa
Source organism: Salmonella enterica subsp. enterica serovar Choleraesuis str. SCSA50
Expression system: Escherichia coli BL21(DE3)
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand BIG 2 x BIG
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 68.05Å b: 46.61Å c: 70.94Å
α: 90° β: 114.78° γ: 90°
R-values:
R R work R free
0.188 0.185 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Interference With Quorum-Sensing Signal Biosynthesis as a Promising Therapeutic Strategy Against Multidrug-Resistant Pathogens.
Fleitas Martínez et al. (2018)
11 other articles

3 mentions without citation

Salmonella enterica MTAN at 1.36 Å resolution: a structure-based design of tailored transition state analogs.
Haapalainen et al. (2013)
2 more