4f2d

X-ray diffraction
2.3Å resolution

Crystal Structure of Escherichia coli L-arabinose Isomerase (ECAI) complexed with Ribitol

Released:
Source organism: Escherichia coli K-12
Entry authors: Manjasetty BA, Burley SK, Almo SC, Chance MR, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-arabinose isomerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 500 amino acids
Theoretical weight: 56.97 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08202 (Residues: 1-500; Coverage: 100%)
Gene names: JW0061, araA, b0062
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A, NSLS BEAMLINE X3A
Spacegroup: P3221
Unit cell:
a: 116.472Å b: 116.472Å c: 214.81Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.212 0.255
Expression system: Escherichia coli BL21(DE3)