X-ray diffraction
2.5Å resolution

Crystal structure of carboxylesterase/phospholipase family protein from Francisella tularensis

Entry authors: Filippova EV, Minasov G, Kuhn M, Wawrzak Z, Shuvalova L, Dubrovska I, Winsor JR, Kiryukhina O, Becker DP, Armoush N, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Abhydrolase_2 domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 246 amino acids
Theoretical weight: 27.44 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q5NI32 (Residues: 1-222; Coverage: 100%)
Gene name: FTT_0258
Sequence domains: Phospholipase/Carboxylesterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P1
Unit cell:
a: 50.997Å b: 64.416Å c: 139.039Å
α: 94.89° β: 90.12° γ: 89.96°
R R work R free
0.204 0.199 0.258
Expression system: Escherichia coli BL21(DE3)