› 4f0r

X-ray diffraction
1.8Å resolution

Crystal structure of an adenosine deaminase homolog from Chromobacterium violaceum (target NYSGRC-019589) bound Zn and 5'-Methylthioadenosine (unproductive complex)

Released:
DOI: 10.2210/pdb4f0r/pdb
PDB entry 4f0r coloured by chain, front view.
PDB entry 4f0r coloured by chain, side view.
PDB entry 4f0r coloured by chain, top view.
Source organism: Chromobacterium violaceum ATCC 12472
Entry authors: Kim J, Vetting MW, Sauder JM, Burley SK, Raushel FM, Bonanno JB, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-homocysteine + H(2)O = S-inosyl-L-homocysteine + NH(3)
S-methyl-5'-thioadenosine + H(2)O = S-methyl-5'-thioinosine + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181857 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-methylthioadenosine/S-adenosylhomocysteine deaminase Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 49.21 KDa
Source organism: Chromobacterium violaceum ATCC 12472
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NZ90 (Residues: 1-439; Coverage: 100%)
Gene names: CV_1032, mtaD
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand GOL 1 x GOL
Ligand MTA 1 x MTA
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P41212
Unit cell:
a: 101.152Å b: 101.152Å c: 88.623Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.199
Expression system: Escherichia coli

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