4exx › Citations

Structural meta-analysis of regular human insulin in pharmaceutical formulations.

Fávero-Retto MP, Palmieri LC, Souza TA, Almeida FC, Lima LM
Eur J Pharm Biopharm 85 1112-21 (2013)
Related entries: 4eww, 4ewx, 4ewz, 4ex0, 4ex1, 4ey1, 4ey9, 4eyd, 4eyn, 4eyp, 4f0n, 4f0o, 4f1a, 4f1b, 4f1c, 4f1d, 4f1f, 4f1g, 4f4t, 4f4v, 4f51, 4f8f, 4fg3

Cited: 11 times
EuropePMC logo PMID: 23692694

Abstract

We have studied regular acting, wild-type human insulin at potency of 100 U/mL from four different pharmaceutical products directly from their final finished formulation by the combined use of mass spectrometry (MS), dynamic light scattering (DLS), small-angle X-ray scattering (SAXS), nuclear magnetic resonance (NMR), and single-crystal protein crystallography (PX). All products showed similar oligomeric assembly in solution as judged by DLS and SAXS measurements. The NMR spectra were compatible with well folded proteins, showing close conformational identity for the human insulin in the four products. Crystallographic assays conducted with the final formulated products resulted in all insulin crystals belonging to the R3 space group with two a dimer in the asymmetric unit, both with the B-chain in the T configuration. Meta-analysis of the 24 crystal structures solved from the four distinct insulin products revealed close similarity between them regardless of variables such as biological origin, product batch, country origin of the product, and analytical approach, revealing a low conformational variability for the converging insulin structural ensemble. We propose the use of MS, SAXS, NMR fingerprint, and PX as a precise chemical and structural proof of folding identity of regular insulin in the final, formulated product.

Reviews citing this publication (2)

  1. A Review of the Microbial Production of Bioactive Natural Products and Biologics. Pham JV, Yilma MA, Feliz A, Majid MT, Maffetone N, Walker JR, Kim E, Cho HJ, Reynolds JM, Song MC, Park SR, Yoon YJ. Front Microbiol 10 1404 (2019)
  2. An Evaluation of the Potential of NMR Spectroscopy and Computational Modelling Methods to Inform Biopharmaceutical Formulations. Pandya A, Howard MJ, Zloh M, Dalby PA. Pharmaceutics 10 (2018)

Articles citing this publication (9)

  1. Identification of putative insulin-like peptides and components of insulin signaling pathways in parasitic platyhelminths by the use of genome-wide screening. Wang S, Luo X, Zhang S, Yin C, Dou Y, Cai X. FEBS J. 281 877-893 (2014)
  2. Identification and functional characterisation of a Schistosoma japonicum insulin-like peptide. Du X, McManus DP, Cai P, Hu W, You H. Parasit Vectors 10 181 (2017)
  3. Serial macromolecular crystallography at ALBA Synchrotron Light Source. Martin-Garcia JM, Botha S, Hu H, Jernigan R, Castellví A, Lisova S, Gil F, Calisto B, Crespo I, Roy-Chowdhury S, Grieco A, Ketawala G, Weierstall U, Spence J, Fromme P, Zatsepin N, Boer DR, Carpena X. J Synchrotron Radiat 29 896-907 (2022)
  4. Identification, expression, and innate immune responses of two insulin-like peptide genes in the razor clam Sinonovacula constricta. Niu D, Wang F, Zhao H, Wang Z, Xie S, Li J. Fish Shellfish Immunol. 51 401-404 (2016)
  5. Identifying signatures of proteolytic stability and monomeric propensity in O-glycosylated insulin using molecular simulation. Hsu WT, Ramirez DA, Sammakia T, Tan Z, Shirts MR. J Comput Aided Mol Des 36 313-328 (2022)
  6. Multi-channel in situ dynamic light scattering instrumentation enhancing biological small-angle X-ray scattering experiments at the PETRA III beamline P12. Falke S, Dierks K, Blanchet C, Graewert M, Cipriani F, Meijers R, Svergun D, Betzel C. J Synchrotron Radiat 25 361-372 (2018)
  7. Progressive endoplasmic reticulum stress over time due to human insulin gene mutation contributes to pancreatic beta cell dysfunction. Amirruddin NS, Tan WX, Tan YS, Gardner DS, Bee YM, Verma CS, Hoon S, Lee KO, Teo AKK. Diabetologia 64 2534-2549 (2021)
  8. Rethinking Protein Drug Design with Highly Accurate Structure Prediction of Anti-CRISPR Proteins. Park HM, Park Y, Vankerschaver J, Van Messem A, De Neve W, Shim H. Pharmaceuticals (Basel) 15 310 (2022)
  9. Structure and function of anhydride-modified forms of human insulin: In silico, in vitro and in vivo studies. Chinisaz M, Ebrahim-Habibi A, Dehpour AR, Yaghmaei P, Parivar K, Moosavi-Movahedi AA. Eur J Pharm Sci 96 342-350 (2017)


Quick links