› 4eqg

X-ray diffraction
1.52Å resolution

Crystal structure of histidine triad nucleotide-binding protein 1 (HINT1) from human complexed with Ala-AMS

Released:
DOI: 10.2210/pdb4eqg/pdb
PDB entry 4eqg coloured by chain, front view.
PDB entry 4eqg coloured by chain, side view.
PDB entry 4eqg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Side chain independent recognition of aminoacyl adenylates by the Hint1 transcription suppressor.
Wang J, Fang P, Schimmel P, Guo M
J Phys Chem B 116 6798-805 (2012)
PMID: 22329685

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156024 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine 5'-monophosphoramidase HINT1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 14.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49773 (Residues: 1-126; Coverage: 100%)
Gene names: HINT, HINT1, PKCI1, PRKCNH1
Sequence domains: HIT domain
Structure domains: HIT-like

Ligands and Environments

2 bound ligands:
Ligand A5A 1 x A5A
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 78.309Å b: 46.374Å c: 64.105Å
α: 90° β: 95.02° γ: 90°
R-values:
R R work R free
0.142 0.141 0.164
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Axonal neuropathy with neuromyotonia: there is a HINT.
Peeters et al. (2017)
2 more

1 mention without citation

A new crystal form of human histidine triad nucleotide-binding protein 1 (hHINT1) in complex with adenosine 5'-monophosphate at 1.38 Å resolution.
Dolot et al. (2012)