› 4eqf

X-ray diffraction
3Å resolution

Trip8b-1a#206-567 interacting with the carboxy-terminal seven residues of HCN2

Released:
DOI: 10.2210/pdb4eqf/pdb
PDB entry 4eqf coloured by chain, front view.
PDB entry 4eqf coloured by chain, side view.
PDB entry 4eqf coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure and stoichiometry of an accessory subunit TRIP8b interaction with hyperpolarization-activated cyclic nucleotide-gated channels.
Bankston JR, Camp SS, DiMaio F, Lewis AS, Chetkovich DM, Zagotta WN
Proc Natl Acad Sci U S A 109 7899-904 (2012)
PMID: 22550182

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131575 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PEX5-related protein Chain: A
Molecule details ›
Chain: A
Length: 365 amino acids
Theoretical weight: 40.97 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8C437 (Residues: 206-567; Coverage: 64%)
Gene names: Pex2, Pex5l, Pex5r, Pxr2
Sequence domains: Tetratricopeptide repeat
Structure domains: Tetratricopeptide repeat domain
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 777 Da
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: O88703 (Residues: 857-863; Coverage: 1%)
Gene names: Bcng2, Hac1, Hcn2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41212
Unit cell:
a: 72.32Å b: 72.32Å c: 146.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.281
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

Neurophysiology of HCN channels: from cellular functions to multiple regulations.
He et al. (2014)
9 more

3 mentions without citation

Structure and stoichiometry of an accessory subunit TRIP8b interaction with hyperpolarization-activated cyclic nucleotide-gated channels.
Bankston et al. (2012)
2 more