› 4egq

X-ray diffraction
2.2Å resolution

Crystal structure of D-alanine-D-alanine ligase B from Burkholderia pseudomallei

Released:
DOI: 10.2210/pdb4egq/pdb
PDB entry 4egq coloured by chain, front view.
PDB entry 4egq coloured by chain, side view.
PDB entry 4egq coloured by chain, top view.
Source organism: Burkholderia pseudomallei 1710b
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-174645 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-alanine--D-alanine ligase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 316 amino acids
Theoretical weight: 33.68 KDa
Source organism: Burkholderia pseudomallei 1710b
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3JNE1 (Residues: 1-312; Coverage: 100%)
Gene names: BURPS1710b_3542, ddl
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P1
Unit cell:
a: 45.389Å b: 66.092Å c: 98.558Å
α: 84.47° β: 80.5° γ: 83.59°
R-values:
R R work R free
0.212 0.21 0.25
Expression system: Escherichia coli

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