› 4eep

X-ray diffraction
1.7Å resolution

Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2

Released:
DOI: 10.2210/pdb4eep/pdb
PDB entry 4eep coloured by chain, front view.
PDB entry 4eep coloured by chain, side view.
PDB entry 4eep coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural tuning of the fluorescent protein iLOV for improved photostability.
Christie JM, Hitomi K, Arvai AS, Hartfield KA, Mettlen M, Pratt AJ, Tainer JA, Getzoff ED
J Biol Chem 287 22295-304 (2012)
PMID: 22573334

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161064 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phototropin-2 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 13.41 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P93025 (Residues: 385-496; Coverage: 12%)
Gene names: At5g58140, CAV1, K21L19.6, KIN7, NPL1, PHOT2
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P43212
Unit cell:
a: 40.502Å b: 40.502Å c: 127.315Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.238
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Super-resolution in solution X-ray scattering and its applications to structural systems biology.
Rambo et al. (2013)
14 more

11 mentions without citation

Optogenetic control of ROS production.
Wojtovich et al. (2014)
10 more