X-ray diffraction
1.9Å resolution

Thermostable phosphite dehydrogenase A176R variant in complex with NAD


Function and Biology Details

Reaction catalysed:
Phosphonate + NAD(+) + H(2)O = phosphate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Phosphonate dehydrogenase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 332 amino acids
Theoretical weight: 36.25 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O69054 (Residues: 1-331; Coverage: 99%)
Gene name: ptxD
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

Cofactor: Ligand NAD 6 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 70.298Å b: 122.263Å c: 134.322Å
α: 90° β: 96.43° γ: 90°
R R work R free
0.202 0.2 0.241
Expression system: Escherichia coli BL21(DE3)