4e5n

X-ray diffraction
1.7Å resolution

Thermostable phosphite dehydrogenase in complex with NAD

Released:

Function and Biology Details

Reaction catalysed:
Phosphonate + NAD(+) + H(2)O = phosphate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphonate dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 330 amino acids
Theoretical weight: 36.04 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O69054 (Residues: 1-330; Coverage: 98%)
Gene name: ptxD
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 90.71Å b: 113.54Å c: 130.19Å
α: 90° β: 100.16° γ: 90°
R-values:
R R work R free
0.212 0.21 0.247
Expression system: Escherichia coli BL21(DE3)