4e5m

X-ray diffraction
1.85Å resolution

Thermostable phosphite dehydrogenase E175A/A176R in complex with NADP

Released:

Function and Biology Details

Reaction catalysed:
Phosphonate + NAD(+) + H(2)O = phosphate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphonate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 35.94 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O69054 (Residues: 1-329; Coverage: 98%)
Gene name: ptxD
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 48.168Å b: 114.013Å c: 58.781Å
α: 90° β: 94.11° γ: 90°
R-values:
R R work R free
0.187 0.186 0.217
Expression system: Escherichia coli BL21(DE3)