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Oxidized (Cu2+) peptidylglycine alpha-hydroxylating monooxygenase (PHM) in complex with hydrogen peroxide (1.98 A)

Source organism: Rattus norvegicus
Biochemical function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen
Biological process: peptide metabolic process
Cellular component: membrane

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EC 4.3.2.5: Peptidylamidoglycolate lyase

Reaction catalysed:
[Peptide]-(2S)-2-hydroxyglycine = [peptide]-amide + glyoxylate
Systematic name:
[Peptide]-(2S)-2-hydroxyglycine peptidylamide-lyase (glyoxylate-forming)
Alternative Name(s):
  • Alpha-hydroxyglycine amidating dealkylase
  • HGAD
  • PAL
  • PGL
  • Peptidyl-alpha-hydroxyglycine alpha-amidating lyase
  • Peptidylamidoglycolate peptidylamide-lyase

EC 1.14.17.3: Peptidylglycine monooxygenase

Reaction catalysed:
[Peptide]-glycine + 2 ascorbate + O(2) = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H(2)O
Systematic name:
[Peptide]-glycine,ascorbate:oxygen oxidoreductase (2-hydroxylating)
Alternative Name(s):
  • PAM
  • PAM-A
  • PAM-B
  • Peptide alpha-amidating enzyme
  • Peptide alpha-amide synthase
  • Peptide-alpha-amide synthetase
  • Peptidyl alpha-amidating enzyme
  • Peptidylglycine 2-hydroxylase
  • Peptidylglycine alpha-amidating monooxygenase
  • Peptidylglycine alpha-hydroxylase

GO terms

Sequence families

Pfam Protein families (Pfam)
PF03712
Domain description: Copper type II ascorbate-dependent monooxygenase, C-terminal domain
Occurring in:
  1. Peptidylglycine alpha-amidating monooxygenase
The deposited structure of PDB entry 4e4z contains 1 copy of Pfam domain PF03712 (Copper type II ascorbate-dependent monooxygenase, C-terminal domain) in Peptidylglycine alpha-amidating monooxygenase. Showing 1 copy in chain A.

PF01082
Domain description: Copper type II ascorbate-dependent monooxygenase, N-terminal domain
Occurring in:
  1. Peptidylglycine alpha-amidating monooxygenase
The deposited structure of PDB entry 4e4z contains 1 copy of Pfam domain PF01082 (Copper type II ascorbate-dependent monooxygenase, N-terminal domain) in Peptidylglycine alpha-amidating monooxygenase. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR000720
Domain description: Peptidylglycine alpha-hydroxylating monooxygenase/peptidyl-hydroxyglycine alpha-amidating lyase
Occurring in:
  1. Peptidylglycine alpha-amidating monooxygenase
IPR008977
Domain description: PHM/PNGase F domain superfamily
Occurring in:
  1. Peptidylglycine alpha-amidating monooxygenase
IPR014783
Domain description: Copper type II, ascorbate-dependent monooxygenase, histidine-cluster-2 conserved site
Occurring in:
  1. Peptidylglycine alpha-amidating monooxygenase
IPR014784
Domain description: Copper type II, ascorbate-dependent monooxygenase-like, C-terminal
Occurring in:
  1. Peptidylglycine alpha-amidating monooxygenase
IPR024548
Domain description: Copper type II ascorbate-dependent monooxygenase, C-terminal
Occurring in:
  1. Peptidylglycine alpha-amidating monooxygenase
IPR020611
Domain description: Copper type II, ascorbate-dependent monooxygenase, histidine-cluster-1 conserved site
Occurring in:
  1. Peptidylglycine alpha-amidating monooxygenase
IPR000323
Domain description: Copper type II, ascorbate-dependent monooxygenase, N-terminal
Occurring in:
  1. Peptidylglycine alpha-amidating monooxygenase
IPR036939
Domain description: Copper type II, ascorbate-dependent monooxygenase, N-terminal domain superfamily
Occurring in:
  1. Peptidylglycine alpha-amidating monooxygenase

Structure domains

CATH CATH domains
2.60.120.230
Class: Mainly Beta
Architecture: Sandwich
Topology: Jelly Rolls
Homology: Jelly Rolls
Occurring in:
  1. Peptidylglycine alpha-amidating monooxygenase
The deposited structure of PDB entry 4e4z contains 1 copy of CATH domain 2.60.120.230 (Jelly Rolls) in Peptidylglycine alpha-amidating monooxygenase. Showing 1 copy in chain A.
2.60.120.310
Class: Mainly Beta
Architecture: Sandwich
Topology: Jelly Rolls
Homology: Copper type II, ascorbate-dependent monooxygenase, N-terminal domain
Occurring in:
  1. Peptidylglycine alpha-amidating monooxygenase
The deposited structure of PDB entry 4e4z contains 1 copy of CATH domain 2.60.120.310 (Jelly Rolls) in Peptidylglycine alpha-amidating monooxygenase. Showing 1 copy in chain A.

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