› 4e45

X-ray diffraction
2Å resolution

Crystal structure of the hMHF1/hMHF2 Histone-Fold Tetramer in Complex with Fanconi Anemia Associated Helicase hFANCM

Released:
DOI: 10.2210/pdb4e45/pdb
PDB 4e45 coloured by chain and viewed from the front.
PDB 4e45 coloured by chain and viewed from the side.
PDB 4e45 coloured by chain and viewed from the top.
Source organism: Homo sapiens
Entry authors: Fox III D, Zhao Y, Yang W, Weidong W

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Centromere protein S Chains: A, C, F, H, K, M
Molecule details ›
Chains: A, C, F, H, K, M
Length: 112 amino acids
Theoretical weight: 13.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N2Z9 (Residues: 1-110; Coverage: 80%)
Gene names: APITD1, CENPS, FAAP16, MHF1
Sequence domains: CENP-S protein
Structure domains: Histone, subunit A
Centromere protein X Chains: B, D, G, I, L, N
Molecule details ›
Chains: B, D, G, I, L, N
Length: 83 amino acids
Theoretical weight: 9.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8MT69 (Residues: 1-81; Coverage: 100%)
Gene names: CENPX, FAAP10, MHF2, STRA13
Sequence domains: CENP-S associating Centromere protein X
Structure domains:
Fanconi anemia group M protein Chains: E, J, O
Molecule details ›
Chains: E, J, O
Length: 137 amino acids
Theoretical weight: 16.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IYD8 (Residues: 667-800; Coverage: 7%)
Gene names: FANCM, KIAA1596
Sequence domains: FANCM to MHF binding domain

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 110.98Å b: 69.97Å c: 116.05Å
α: 90° β: 91.6° γ: 90°
R-values:
R R work R free
0.202 0.202 0.245
Expression system: Escherichia coli BL21(DE3)

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