PDBe 4e45

X-ray diffraction
2Å resolution

Crystal structure of the hMHF1/hMHF2 Histone-Fold Tetramer in Complex with Fanconi Anemia Associated Helicase hFANCM

Released:
Source organism: Homo sapiens
Entry authors: Fox III D, Zhao Y, Yang W, Weidong W

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Centromere protein S Chains: A, C, F, H, K, M
Molecule details ›
Chains: A, C, F, H, K, M
Length: 112 amino acids
Theoretical weight: 13.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N2Z9 (Residues: 1-110; Coverage: 80%)
Gene names: APITD1, CENPS, FAAP16, MHF1
Sequence domains: CENP-S protein
Structure domains: Histone, subunit A
Centromere protein X Chains: B, D, G, I, L, N
Molecule details ›
Chains: B, D, G, I, L, N
Length: 83 amino acids
Theoretical weight: 9.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8MT69 (Residues: 1-81; Coverage: 100%)
Gene names: CENPX, FAAP10, MHF2, STRA13
Sequence domains: CENP-S associating Centromere protein X
Structure domains: GTP Cyclohydrolase I; Chain A, domain 1
Fanconi anemia group M protein Chains: E, J, O
Molecule details ›
Chains: E, J, O
Length: 137 amino acids
Theoretical weight: 16.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IYD8 (Residues: 667-800; Coverage: 7%)
Gene names: FANCM, KIAA1596
Sequence domains: FANCM to MHF binding domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 110.98Å b: 69.97Å c: 116.05Å
α: 90° β: 91.6° γ: 90°
R-values:
R R work R free
0.202 0.202 0.245
Expression system: Escherichia coli BL21(DE3)