4e44

X-ray diffraction
2.1Å resolution

Crystal structure of the hMHF1/hMHF2 Histone-Fold Tetramer

Released:
Source organism: Homo sapiens
Entry authors: Fox III D, Zhao Y, Yang W, Weidong W

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Centromere protein S Chains: A, C
Molecule details ›
Chains: A, C
Length: 112 amino acids
Theoretical weight: 13.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N2Z9 (Residues: 1-110; Coverage: 80%)
Gene names: APITD1, CENPS, FAAP16, MHF1
Sequence domains: CENP-S protein
Structure domains: Histone, subunit A
Centromere protein X Chains: B, D
Molecule details ›
Chains: B, D
Length: 83 amino acids
Theoretical weight: 9.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8MT69 (Residues: 1-81; Coverage: 100%)
Gene names: CENPX, FAAP10, MHF2, STRA13
Sequence domains: CENP-S associating Centromere protein X
Structure domains: GTP Cyclohydrolase I; Chain A, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 40.78Å b: 91.93Å c: 61.43Å
α: 90° β: 96.3° γ: 90°
R-values:
R R work R free
0.2 0.2 0.231
Expression system: Escherichia coli BL21(DE3)