› 4dx3

X-ray diffraction
1.65Å resolution

Crystal structure of an enolase (mandelate racemase subgroup, target EFI-502086) from Agrobacterium tumefaciens, with a succinimide residue

Released:
DOI: 10.2210/pdb4dx3/pdb
PDB 4dx3 coloured by chain and viewed from the front.
PDB 4dx3 coloured by chain and viewed from the side.
PDB 4dx3 coloured by chain and viewed from the top.
Source organism: Agrobacterium fabrum str. C58
Entry authors: Vetting MW, Bouvier JT, Toro R, Bhosle R, Al Obaidi NF, Morisco LL, Wasserman SR, Sojitra S, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MR_MLE domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 44.08 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7D1T6 (Residues: 1-400; Coverage: 100%)
Gene name: Atu0270
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand CL 1 x CL
1 modified residue:
Ligand SNN 1 x SNN

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I422
Unit cell:
a: 114.54Å b: 114.54Å c: 129.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.172
Expression system: Escherichia coli BL21(DE3)

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