› 4dvc

X-ray diffraction
1.2Å resolution

Structural and functional studies of TcpG, the Vibrio cholerae DsbA disulfide-forming protein required for pilus and cholera toxin production

Released:
DOI: 10.2210/pdb4dvc/pdb
PDB entry 4dvc coloured by chain, front view.
PDB entry 4dvc coloured by chain, side view.
PDB entry 4dvc coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Primary publication:
The 1.2 Å resolution crystal structure of TcpG, the Vibrio cholerae DsbA disulfide-forming protein required for pilus and cholera-toxin production.
Walden PM, Heras B, Chen KE, Halili MA, Rimmer K, Sharma P, Scanlon MJ, Martin JL
Acta Crystallogr D Biol Crystallogr 68 1290-302 (2012)
PMID: 22993083

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152357 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol:disulfide interchange protein DsbA Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.75 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
UniProt:
  • Canonical: P32557 (Residues: 19-200; Coverage: 100%)
Gene names: VC_0034, dsbA, tpcG
Sequence domains: DSBA-like thioredoxin domain
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
Ligand DMS 2 x DMS
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2221
Unit cell:
a: 62.654Å b: 89.52Å c: 64.263Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.12 0.119 0.139
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Four structural subclasses of the antivirulence drug target disulfide oxidoreductase DsbA provide a platform for design of subclass-specific inhibitors.
McMahon et al. (2014)
2 more

2 mentions without citation

Phylodynamic analysis of clinical and environmental Vibrio cholerae isolates from Haiti reveals diversification driven by positive selection.
Azarian et al. (2014)
1 more