4dux

X-ray diffraction
2.3Å resolution

E. coli (lacZ) beta-galactosidase (N460S) in complex with L-ribose

Released:
Source organism: Escherichia coli K-12
Entry authors: Wheatley RW, Lo S, Janzcewicz LJ, Dugdale ML, Huber RE

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1052 amino acids
Theoretical weight: 119.72 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00722 (Residues: 10-1024; Coverage: 99%)
Gene names: JW0335, b0344, lacZ
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P212121
Unit cell:
a: 150.648Å b: 168.103Å c: 201.881Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.17 0.221
Expression system: Escherichia coli