X-ray diffraction
2.1Å resolution

E. coli (lacZ) beta-galactosidase (G974A) 2-deoxy-galactosyl-enzyme and bis-Tris complex

Source organism: Escherichia coli K-12
Entry authors: Wheatley RW, Lo S, Janzcewicz LJ, Dugdale ML, Huber RE

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Beta-galactosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1052 amino acids
Theoretical weight: 119.76 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P00722 (Residues: 10-1024; Coverage: 99%)
Gene names: JW0335, b0344, lacZ
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P212121
Unit cell:
a: 150.57Å b: 167.731Å c: 201.64Å
α: 90° β: 90° γ: 90°
R R work R free
0.159 0.158 0.206
Expression system: Escherichia coli