4dui

X-ray diffraction
1.16Å resolution

DARPIN D1 binding to tubulin beta chain (not in complex)

Released:
Source organism: synthetic construct
Entry authors: Pecqueur L, Duellberg C, Dreier B, Wang Q, Jiang C, Pluckthun A, Surrey T, Gigant B, Knossow M

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DESIGNED ANKYRIN REPEAT PROTEIN (DARPIN) D1 Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 18.08 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P41212
Unit cell:
a: 43.4Å b: 43.4Å c: 143.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.125 0.123 0.149
Expression system: Escherichia coli