4dtm

X-ray diffraction
1.95Å resolution

RB69 DNA Polymerase Ternary Complex with dCTP Opposite an Abasic Site and ddG/dC as the Penultimate Base-pair

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-directed DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 901 amino acids
Theoretical weight: 104.23 KDa
Source organism: Escherichia phage RB69
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38087 (Residues: 1-901; Coverage: 100%)
Gene name: 43
Sequence domains:
Structure domains:
DNA template Chain: T
Molecule details ›
Chain: T
Length: 18 nucleotides
Theoretical weight: 5.37 KDa
Source organism: Escherichia phage RB69
Expression system: Not provided
DNA primer Chain: P
Molecule details ›
Chain: P
Length: 13 nucleotides
Theoretical weight: 4.01 KDa
Source organism: Escherichia phage RB69
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 75.178Å b: 120.499Å c: 130.752Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.22
Expression systems:
  • Escherichia coli
  • Not provided