4ds8

X-ray diffraction
2.21Å resolution

Complex structure of abscisic acid receptor PYL3-(+)-ABA-HAB1 in the presence of Mn2+

Released:

Function and Biology Details

Reaction catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Abscisic acid receptor PYL3 Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 23.32 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SSM7 (Residues: 1-209; Coverage: 100%)
Gene names: At1g73000, F3N23.20, PYL3, RCAR13
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4
Protein phosphatase 2C 16 Chain: B
Molecule details ›
Chain: B
Length: 343 amino acids
Theoretical weight: 38.13 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9CAJ0 (Residues: 169-511; Coverage: 70%)
Gene names: At1g72770, F28P22.4, HAB1, P2C-HA
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 39.745Å b: 69.723Å c: 82.887Å
α: 90° β: 101.63° γ: 90°
R-values:
R R work R free
0.188 0.186 0.221
Expression system: Escherichia coli