X-ray diffraction
2.38Å resolution

Quadruple mutant (N51I+C59R+S108N+I164L) Plasmodium falciparum dihydrofolate reductase-thymidylate synthase (PfDHFR-TS) complexed with P65 and NADPH


Function and Biology Details

Reactions catalysed:
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Bifunctional dihydrofolate reductase-thymidylate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 608 amino acids
Theoretical weight: 71.91 KDa
Source organism: Plasmodium falciparum VS/1
Expression system: Escherichia coli
  • Canonical: D9N170 (Residues: 1-608; Coverage: 100%)
Gene names: DHFR-TS, V1/S
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand NDP 2 x NDP
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Cu FINE FOCUS
Spacegroup: P212121
Unit cell:
a: 57.951Å b: 156.447Å c: 164.653Å
α: 90° β: 90° γ: 90°
R R work R free
0.222 0.219 0.286
Expression system: Escherichia coli