› 4dow

X-ray diffraction
1.95Å resolution

Structure of mouse ORC1 BAH domain bound to H4K20me2

Released:
DOI: 10.2210/pdb4dow/pdb
PDB entry 4dow coloured by chain, front view.
PDB entry 4dow coloured by chain, side view.
PDB entry 4dow coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The BAH domain of ORC1 links H4K20me2 to DNA replication licensing and Meier-Gorlin syndrome.
Kuo AJ, Song J, Cheung P, Ishibe-Murakami S, Yamazoe S, Chen JK, Patel DJ, Gozani O
Nature 484 115-9 (2012)
PMID: 22398447

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158702 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Origin recognition complex subunit 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 18.95 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Z1N2 (Residues: 9-170; Coverage: 19%)
Gene names: Orc1, Orc1l
Sequence domains: BAH domain
Structure domains: SH3 type barrels.
Histone H4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 amino acids
Theoretical weight: 1.48 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P62806 (Residues: 15-26; Coverage: 12%)
Gene names: H4-12, H4-53, H4c1, H4c11, H4c12, H4c14, H4c16, H4c2, H4c3, H4c4, H4c6, H4c8, H4c9, H4f16, Hist1h4a, Hist1h4b, Hist1h4c, Hist1h4d, Hist1h4f, Hist1h4h, Hist1h4i, Hist1h4j, Hist1h4k, Hist1h4m, Hist2h4, Hist2h4a, Hist4h4

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLY 2 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P1
Unit cell:
a: 35.125Å b: 49.434Å c: 54.376Å
α: 89.93° β: 102.12° γ: 103.25°
R-values:
R R work R free
0.213 0.21 0.257
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

81 review citations

DNA methylation pathways and their crosstalk with histone methylation.
Du et al. (2015)
80 more

10 mentions without citation

Perceiving the epigenetic landscape through histone readers.
Musselman et al. (2012)
9 more