4doq

X-ray diffraction
2Å resolution

Crystal structure of the complex of Porcine Pancreatic Trypsin with 1/2SLPI

Released:
Source organisms:
Primary publication:
Structure basis 1/2SLPI and porcine pancreas trypsin interaction.
OpenAccess logo J Synchrotron Radiat 20 943-7 (2013)
PMID: 24121345

Function and Biology Details

Reaction catalysed:
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Trypsin Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 223 amino acids
Theoretical weight: 23.49 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00761 (Residues: 9-231; Coverage: 97%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Antileukoproteinase Chains: B, D
Molecule details ›
Chains: B, D
Length: 47 amino acids
Theoretical weight: 5.14 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P03973 (Residues: 85-131; Coverage: 44%)
Gene names: SLPI, WAP4, WFDC4
Sequence domains: WAP-type (Whey Acidic Protein) 'four-disulfide core'
Structure domains: Elafin-like

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 40.535Å b: 118.61Å c: 93.393Å
α: 90° β: 90.74° γ: 90°
R-values:
R R work R free
0.19 0.187 0.242
Expression system: Not provided