4dnr

X-ray diffraction
3.68Å resolution

Crystal structure of the CusBA heavy-metal efflux complex from Escherichia coli, E716F mutant

Released:
Source organism: Escherichia coli K-12
Entry authors: Su C-C, Long F, Yu E

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cation efflux system protein CusB Chains: B, C
Cation efflux system protein CusA Chain: A
Molecule details ›
Chain: A
Length: 1054 amino acids
Theoretical weight: 115.79 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P38054 (Residues: 1-1047; Coverage: 100%)
Gene names: JW0564, b0575, cusA, ybdE
Sequence domains: AcrB/AcrD/AcrF family

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R32
Unit cell:
a: 159.594Å b: 159.594Å c: 689.652Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.246 0.244 0.272
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli