X-ray diffraction
2.15Å resolution

Crystal structure of an amidohydrolase (cog3618) from burkholderia multivorans (target efi-500235) with bound hepes, space group p3221

Entry authors: Vetting MW, Toro R, Bhosle R, Seidel RD, Hillerich B, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Al Obaidi NF, Zencheck WD, Imker HJ, Gerlt JA, Raushel FM, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Amidohydro-rel domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 33.96 KDa
Source organism: Burkholderia multivorans ATCC 17616
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A0A0H3KNC4 (Residues: 1-296; Coverage: 100%)
Gene name: BMULJ_04915
Sequence domains: Amidohydrolase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3221
Unit cell:
a: 75.07Å b: 75.07Å c: 142.26Å
α: 90° β: 90° γ: 120°
R R work R free
0.192 0.19 0.228
Expression system: Escherichia coli BL21(DE3)