› 4dnc

X-ray diffraction
2.05Å resolution

Crystal structure of human MOF in complex with MSL1

Released:
DOI: 10.2210/pdb4dnc/pdb
PDB entry 4dnc coloured by chain, front view.
PDB entry 4dnc coloured by chain, side view.
PDB entry 4dnc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into the regulation of MOF in the male-specific lethal complex and the non-specific lethal complex.
Huang J, Wan B, Wu L, Yang Y, Dou Y, Lei M
Cell Res 22 1078-81 (2012)
PMID: 22547026

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-179330 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone acetyltransferase KAT8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 33.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H7Z6 (Residues: 170-458; Coverage: 63%)
Gene names: KAT8, MOF, MYST1, PP7073
Sequence domains:
Structure domains:
Male-specific lethal 1 homolog Chains: D, E
Molecule details ›
Chains: D, E
Length: 48 amino acids
Theoretical weight: 5.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q68DK7 (Residues: 473-520; Coverage: 8%)
Gene names: MSL1, MSL1L1
Sequence domains: PEHE domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand ALY 2 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 64.801Å b: 111.518Å c: 66.751Å
α: 90° β: 111.05° γ: 90°
R-values:
R R work R free
0.212 0.208 0.246
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Molecular Basis for Histone Acetyltransferase Regulation by Binding Partners, Associated Domains, and Autoacetylation.
McCullough et al. (2016)
1 more

1 mention without citation

Structural insight into the regulation of MOF in the male-specific lethal complex and the non-specific lethal complex.
Huang et al. (2012)