› 4dll

X-ray diffraction
2.11Å resolution

Crystal structure of a 2-hydroxy-3-oxopropionate reductase from Polaromonas sp. JS666

Released:
DOI: 10.2210/pdb4dll/pdb
PDB entry 4dll coloured by chain, front view.
PDB entry 4dll coloured by chain, side view.
PDB entry 4dll coloured by chain, top view.
Source organism: Polaromonas sp. JS666
Entry authors: Agarwal R, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, Lafleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
D-glycerate + NAD(P)(+) = 2-hydroxy-3-oxopropanoate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171474 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-hydroxy-3-oxopropionate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 320 amino acids
Theoretical weight: 34.48 KDa
Source organism: Polaromonas sp. JS666
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12CU4 (Residues: 1-298; Coverage: 100%)
Gene name: Bpro_1712
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 26 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 39.868Å b: 47.01Å c: 77.498Å
α: 92.14° β: 95.3° γ: 101.19°
R-values:
R R work R free
0.221 0.218 0.278
Expression system: Escherichia coli BL21(DE3)

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