4dl9

X-ray diffraction
1.85Å resolution

Crystal structure of S-nitrosoglutathione reductase from tomato (Solanum lycopersicum) in complex with NAD+

Released:
Source organism: Solanum lycopersicum
Entry authors: Kopecny D, Tylichova M, Briozzo P

Function and Biology Details

Reaction catalysed:
S-(hydroxymethyl)glutathione + NAD(P)(+) = S-formylglutathione + NAD(P)H
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-(hydroxymethyl)glutathione dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 42.58 KDa
Source organism: Solanum lycopersicum
Expression system: Escherichia coli
UniProt:
  • Canonical: D2Y3F4 (Residues: 2-379; Coverage: 100%)
Gene names: 100750249, ADH3
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 82.114Å b: 125.076Å c: 76.761Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.218
Expression system: Escherichia coli