X-ray diffraction
1.4Å resolution

Human mesotrypsin-S39Y complexed with bovine pancreatic trypsin inhibitor (BPTI)


Function and Biology Details

Reaction catalysed:
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Trypsin-3 Chains: A, B, D, G
Molecule details ›
Chains: A, B, D, G
Length: 224 amino acids
Theoretical weight: 24.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P35030 (Residues: 81-304; Coverage: 74%)
Gene names: PRSS3, PRSS4, TRY3, TRY4
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Pancreatic trypsin inhibitor Chains: C, E, F, H
Molecule details ›
Chains: C, E, F, H
Length: 58 amino acids
Theoretical weight: 6.53 KDa
Source organism: Bos taurus
Expression system: Komagataella pastoris
  • Canonical: P00974 (Residues: 36-93; Coverage: 73%)
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21
Unit cell:
a: 73.529Å b: 109.949Å c: 81.116Å
α: 90° β: 116.82° γ: 90°
R R work R free
0.166 0.166 0.211
Expression systems:
  • Escherichia coli
  • Komagataella pastoris