X-ray diffraction
3Å resolution

Crystal structure of R194A mutant of cAMP-dependent protein kinase with unphosphorylated activation loop


Function and Biology Details

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Entry contents:
1 distinct polypeptide molecule
cAMP-dependent protein kinase catalytic subunit alpha Chains: A, E
Molecule details ›
Chains: A, E
Length: 371 amino acids
Theoretical weight: 42.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
  • Canonical: P05132 (Residues: 1-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3221
Unit cell:
a: 95.745Å b: 95.745Å c: 173.959Å
α: 90° β: 90° γ: 120°
R R work R free
0.253 0.252 0.286
Expression system: Escherichia coli