› 4dct

X-ray diffraction
2.3Å resolution

Crystal Structure of B. subtilis EngA in complex with half-occupacy GDP

Released:
DOI: 10.2210/pdb4dct/pdb
PDB 4dct coloured by chain and viewed from the front.
PDB 4dct coloured by chain and viewed from the side.
PDB 4dct coloured by chain and viewed from the top.
Source organism: Bacillus subtilis
Primary publication:
Potassium Acts as a GTPase-Activating Element on Each Nucleotide-Binding Domain of the Essential Bacillus subtilis EngA.
Foucher AE, Reiser JB, Ebel C, Housset D, Jault JM
PLoS ONE 7 e46795 (2012)
PMID: 23056455

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase Der Chain: A
Molecule details ›
Chain: A
Length: 456 amino acids
Theoretical weight: 51 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P50743 (Residues: 1-436; Coverage: 100%)
Gene names: BSU22840, der, engA, yphC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 62.76Å b: 65.26Å c: 112.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.224 0.295
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Invited review: MnmE, a GTPase that drives a complex tRNA modification reaction.
Fislage et al. (2016)
2 more

33 mentions without citation

Advances in the use of motion management and image guidance in radiation therapy treatment for lung cancer.
Molitoris et al. (2018)
32 more