X-ray diffraction
2.81Å resolution

Crystal Structure of F189W Actinorhodin Polyketide Ketoreductase with NADPH


Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Putative ketoacyl reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 281 amino acids
Theoretical weight: 29.5 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P16544 (Residues: 1-261; Coverage: 100%)
Gene names: SCBAC28G1.12c, SCO5086, actIII
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3221
Unit cell:
a: 103.527Å b: 103.527Å c: 123.232Å
α: 90° β: 90° γ: 120°
R R work R free
0.191 0.188 0.246
Expression system: Escherichia coli BL21(DE3)