› 4dbf

X-ray diffraction
1.9Å resolution

Crystal structures of Cg1458

Released:
DOI: 10.2210/pdb4dbf/pdb
PDB entry 4dbf coloured by chain, front view.
PDB entry 4dbf coloured by chain, side view.
PDB entry 4dbf coloured by chain, top view.
Source organism: Corynebacterium glutamicum
Primary publication:
Crystal structures of Cg1458 reveal a catalytic lid domain and a common catalytic mechanism for the FAH family.
Ran T, Gao Y, Marsh M, Zhu W, Wang M, Mao X, Xu L, Xu D, Wang W
Biochem J 449 51-60 (2013)
PMID: 23046410

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185642 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (Catechol pathway) Chains: A, B
Molecule details ›
Chains: A, B
Length: 288 amino acids
Theoretical weight: 31.2 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NQY2 (Residues: 1-268; Coverage: 100%)
Gene name: Cgl1290
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 124.12Å b: 124.12Å c: 73.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.244
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The fumarylacetoacetate hydrolase (FAH) superfamily of enzymes: multifunctional enzymes from microbes to mitochondria.
Weiss et al. (2018)
1 more

2 mentions without citation

Structural basis for the bi-functionality of human oxaloacetate decarboxylase FAHD1.
Weiss et al. (2018)
1 more