4db4

X-ray diffraction
3.6Å resolution

Mss116p DEAD-box helicase domain 2 bound to a chimaeric RNA-DNA duplex

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
ATP-dependent RNA helicase MSS116, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 29.13 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P15424 (Residues: 342-596; Coverage: 38%)
Gene names: MSS116, YD9346.05C, YDR194C
Sequence domains: Helicase conserved C-terminal domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases
5'-R(*GP*GP*GP*CP*GP*GP*G)-D(P*CP*CP*CP*GP*CP*CP*C)-3' Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 14 nucleotides
Theoretical weight: 4.4 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 43.696Å b: 70.112Å c: 214.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.245 0.28
Expression systems:
  • Escherichia coli BL21
  • Not provided