4d87

X-ray diffraction
3.5Å resolution

Crystal Structure of Tyrosinase from Bacillus megaterium in complex with SDS

Released:
Source organism: Priestia megaterium
Entry authors: Adir N, Goldfeder M, Fishman A

Function and Biology Details

Reaction catalysed:
(1a) L-tyrosine + 1/2 O(2) = L-dopa
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 35.28 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: B2ZB02 (Residues: 1-297; Coverage: 100%)
Sequence domains: Common central domain of tyrosinase
Structure domains: Di-copper center containing domain from catechol oxidase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 47.84Å b: 79.14Å c: 85.93Å
α: 90° β: 103.56° γ: 90°
R-values:
R R work R free
0.262 0.259 0.293
Expression system: Escherichia coli