4d7e

X-ray diffraction
2.4Å resolution

An unprecedented NADPH domain conformation in Lysine Monooxygenase NbtG from Nocardia farcinica

Released:

Function and Biology Details

Reaction catalysed:
L-lysine + NADPH + O(2) = N(6)-hydroxy-L-lysine + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lysine N6-monooxygenase MbtG Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 429 amino acids
Theoretical weight: 46.6 KDa
Source organism: Nocardia farcinica IFM 10152
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5Z1T5 (Residues: 1-429; Coverage: 100%)
Gene names: NFA_7610, nbtG
Sequence domains: L-lysine 6-monooxygenase (NADPH-requiring)

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P21
Unit cell:
a: 80.64Å b: 121.92Å c: 91.86Å
α: 90° β: 97.72° γ: 90°
R-values:
R R work R free
0.211 0.208 0.281
Expression system: Escherichia coli