X-ray diffraction
3.16Å resolution

human PFKFB3 in complex with a pyrrolopyrimidone compound

Source organism: Homo sapiens
Entry authors: Stgallay SA, Bennett N, Critchlow S, Davies G, Debreczeni JE, Evans N, Holdgate G, Jones NP, Leach L, Maman S, McLoughlin S, Preston M, Rigoreau L, Thomas A, Walker G, Walsch J, Ward RA, Wheatley E, Winter J

Function and Biology Details

Reactions catalysed:
Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 Chain: A
Molecule details ›
Chain: A
Length: 449 amino acids
Theoretical weight: 52.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
  • Canonical: Q16875 (Residues: 1-449; Coverage: 86%)
Gene name: PFKFB3
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P6522
Unit cell:
a: 102.28Å b: 102.28Å c: 256.89Å
α: 90° β: 90° γ: 120°
R R work R free
0.208 0.207 0.219
Expression system: Escherichia coli BL21