X-ray diffraction
1.3Å resolution

The catalytic domain, BcGH76, of Bacillus circulans Aman6 in complex with 1,6-ManDMJ


Function and Biology Details

Reaction catalysed:
Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
CBM6 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 40.93 KDa
Source organism: Niallia circulans
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9Z4P9 (Residues: 35-375; Coverage: 61%)
Gene name: aman6
Sequence domains: Glycosyl hydrolase family 76
Structure domains: Glycosyltransferase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 82.248Å b: 85.285Å c: 100.613Å
α: 90° β: 90° γ: 90°
R R work R free
0.177 0.175 0.209
Expression system: Escherichia coli BL21(DE3)