› 4d40

X-ray diffraction
1.67Å resolution

High-Resolution Structure of a Type IV Pilin from Shewanella oneidensis

Released:
DOI: 10.2210/pdb4d40/pdb
PDB entry 4d40 coloured by chain, front view.
PDB entry 4d40 coloured by chain, side view.
PDB entry 4d40 coloured by chain, top view.
Source organism: Shewanella oneidensis MR-1
Primary publication:
High-resolution structure of a type IV pilin from the metal-reducing bacterium Shewanella oneidensis.
Gorgel M, Ulstrup JJ, Bøggild A, Jones NC, Hoffmann SV, Nissen P, Boesen T
BMC Struct Biol 15 4 (2015)
PMID: 25886849

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PilD processed protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 89 amino acids
Theoretical weight: 9.91 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8EII5 (Residues: 36-123; Coverage: 72%)
Gene name: SO_0854
Sequence domains: Type IV minor pilin ComP, DNA uptake sequence receptor

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: I222
Unit cell:
a: 48.91Å b: 96.46Å c: 110.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Motility and adhesion through type IV pili in Gram-positive bacteria.
Piepenbrink et al. (2016)
1 more

2 mentions without citation

High-resolution structure of a type IV pilin from the metal-reducing bacterium Shewanella oneidensis.
Gorgel et al. (2015)
1 more